Compatibility of Agilent Jet Stream thermal gradient focusing technology with CE/MS

Coupling capillary electrophoresis to mass spectrometers through the Agilent triple tube coaxial sheath-fl ow sprayer is a widely used confi guration due to its simplicity, versatility, and robustness. This Technical Overview shows that with the Jet Stream source and the latest version of the CE/MS sprayer, this approach can now also be used in combination with the proprietary Agilent Jet Stream thermal gradient focusing technology. The sprayer worked as well on a mass spectrometer equipped with a conventional ESI source and showed, in comparison to the standard sprayer, a slightly increased performance. When the sprayer was used in combination with an Agilent Jet Stream source, equivalent or better results were obtained as compared to a conventional ESI source. Authors Isabelle Kohler, Julie Schappler, Serge Rudaz School of Pharmaceutical Sciences University of Geneva Geneva, Switzerland Hans-Peter Zimmermann, Christian Wenz Agilent Technologies, Inc. Waldbronn, Germany